Affymetrix expression manual

Affymetrix is dedicated to helping you design and analyze GeneChip® expression profiling experiments that generate high-quality, statistically sound, and biologically interesting results. This guide provides information, resources, and tools to help you easily design and analyze experiments and maximize the value derived from your GeneChip data. 2 Motivation The Affymetrix spike-in study1 is a subset of the data used to develop and validate the MAS expression measure algorithm, Affymetrix’s current default (Affymetrix, ). For this experi- ment, human cRNA fragments matching 16 probe-sets on one of the Affymetrix human chips, the HGU95A GeneChip, were added to a hybridization. regulators of gene expression, and the widespread changes in gene expression seen in drug abuse (Albertson et al. ; Lehrmann and Freed ), we developed a computational and manual annotation pipeline to identify additional lncRNA transcripts represented on Affymetrix arrays. We went on to determine the lncRNAs expressed in human.

3' IVT Express Kit User Manual (pdf, MB) Affymetrix® Human SNP Assay User Guide for Automated Target Preparation (pdf, MB) Ambion® WT Expression Kit (pdf, MB) Cytogenetics Assay Protocol (pdf, MB) Expression Analysis Technical Manual - Cartridge Arrays Using the Array Station (pdf, MB). evaluated by Affymetrix scientists. Information and part numbers listed are based on U.S. catalog information. For supplier information, please refer to th e Supplier Reference List in Appendix A of this manual. Total RNA Isolation TRIzol Reagent, Invitrogen Life Technologies, P/N RNeasy Mini Kit, QIAGEN, P/N Poly-A mRNA Isolation. Complete Manual: Expression Analysis Technical Manual (with hyperlinks) (zip, MB) Expression Analysis Technical Manual (for print) (zip, KB) Sections 1 - 4: (Manual Sections) Section 1: GeneChip Expression Analysis Overview. (pdf, KB) Section 2: Eukaryotic Sample and Array Processing.

Download manuals for Affymetrix products, including: NetAffx Analysis Center User's Guide (pdf, 1 MB); Expression Analysis Technical Manual (pdf. This guide gives a tutorial-style introduction to the main limma features but does not for assessing differential expression in microarray experiments. GeneSpring expression analysis: This module enables the following gene expression analysis workflows: – Affymetrix® 3' IVT arrays.